The former world number one had been due to step on court for his opening round match against Spaniard Albert Montanes later on Sunday.
The 30-year-old South Australian's latest complaint puts in doubt his chances of competing at Wimbledon next month.
The two-time grand slam champion had been on the mend from foot surgery and was due to play his first match in two months against Montanes.
The official reason given for his withdrawal was a left ankle problem, but it is believed he suffered a flare-up of a toe injury.
Whatever the problem, the 30-year-old's fragile body is creaking following years of toil on the professional circuit.
Hewitt's camp had set modest expectations for him at the French Open in his return to the court following foot surgery two months ago.
His coach Tony Roche said pre-tournament that the major focus had been on him gaining fitness for Wimbledon.
It is understood that Hewitt is still intending on defending his title on grass in Halle in a fortnight's time.
Hewitt's camp was unable to be contacted for comment.
His withdrawal leaves Bernard Tomic as the nation's only player left in the men's singles draw in Paris, with the teenage wildcard in action on Monday against Argentine Carlos Berlocq.
-AAPTags: sport, tennis, australia, france First posted May 22, 2011 18:52:00